Synergistic toxic effects of citrinin, ochratoxin A and penicillic acid in mice.

G. A. SANSING, E. B. LrLLEHOJ, R. W. DHROY and M. A. MILLER. Synergistic toxic effects of citrinin, ochratoxin A and penicillic acid in mice. Toxicoll 14, 213-220, 1976.-The LD50'S in mice of citrinin, ochratoxin A, and penicillic acid injected intraperitone,"lIy were 89,22, and 100 mg per kg of body weight, respectively. Paired combinations of the mycotoxins, citrinin: ochratoxin A (Cl:OA), ochratoxin A:penicillic acid (OA:PA), and penicillic acid:citrinin (PA :CI) elicited synergistic lethal responses. After administration of citrinin, 1"C-orotic acid incorporation into both liver and kidney increased significantly by 27 hI' with a return to control levels at 51 hI'. Treatment with penicillic acid also increased orotic acid incorporation into liver ribonucleic acid (RNA) at 27 hI' and at 4 hI' in the kidney. Ochratoxin A inhibited orotic acid incorporation into both liver and kidney RNA 6 hI' after toxin injection with a subsequent return to control levels at 27 hr. Orotic acid incorporation was inhibited in both kidney and liver 6 hI' after treatment with the toxin combination Cl :OA. PA :CI stimulated precursor incorporation into kidney RNA at 27 hI' and inhibited the function in liver. The OA :PA combination inhibited orotic acid incorporation in both organs 15-27 hI' after toxin treatment.